She was intended to be voiced by Mary Jo Catlett, who voices her in the show.
In the show
In the movie
Originally, it was planned for The SpongeBob Movie: Sponge Out of Water to include an scene after Spongebob and Plankton return to Bikini Bottom, which has turned into an apocalyptic state following the disapperance of the Krabby Patty formula thanks to Burger-Beard's machinations. In the scene, an skull-masked driver chases Spongebob and Plankton in a boatmobile chase with a monster boattruck. The song "Squeeze Me" plays out on Plankton's radio until the boat crashes. As a result, the masked driver reveals herself to be a disguised Mrs. Puff and warns SpongeBob that he will pay the ultimate penalty for his reckless driving, taking her bag full of torture devices and taking a red pen, which she uses to give SpongeBob a failing grade.
However, the scene had to be deleted due the directives of Paramount Pictures, likely because it was unsuitable for kids, depicting an extremely dangerous situation which ends with a silly resolution. The animatics of the deleted scene was included in the film's Blu-ray release. In the finished film, Mrs. Puff still makes an appearance, but with her normal clothes instead of apocalyptic ones and in a cameo role.
- It's speculated that another reason for which this scene may have been deleted was to not depict Mrs. Puff's infamous flanderized role depicted in the episodes following The SpongeBob SquarePants Movie until the late Stephen Hillenburg returned to revisit the show.
- Interestingly, had this scene been kept, this would have been the second time Mrs. Puff tried to murder SpongeBob with a boattruck, as she previously tried to kill him with such method in the episode "Demolition Doofus", which namely led her to cross the Moral Event Horizon and to reach the highest peak of her above mentioned controversial antagonistic side.